ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of high temperature stress on the root vitality and leaf biochemical indexes in populations of Pinellia ternate.</p><p><b>METHOD</b>The leaf activity of SOD, contents of MDA and free praline and root vitality were determined after a treatment of high temperature stress (35 degrees C/25 degrees C) was given to different populations of P. ternate.</p><p><b>RESULT AND CONCLUSION</b>By stress time increasing, the leaf SOD and free praline rose firstly and then dropped, the content of MDA increased while the root ability decreased in all populations. And the response of populations of P. ternate to high temperature was significantly different.</p>
Subject(s)
China , Ecosystem , Heat-Shock Response , Physiology , Hot Temperature , Malondialdehyde , Metabolism , Pinellia , Metabolism , Physiology , Plant Leaves , Metabolism , Physiology , Plant Roots , Physiology , Plants, Medicinal , Metabolism , Physiology , Proline , Metabolism , Superoxide Dismutase , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To analyze the content of guanosine, total alkaloid and individual yield of Pinellia ternata from different populations in China and evaluate its quality.</p><p><b>METHOD</b>Reversed-phase high-performance liquid chromatography (HPLC) was used to determine the content of guanosine. The content of alkaloid was determined by ultra violet spectrophometry. The results were analyzed by SPSS software.</p><p><b>RESULT</b>The contents of guanosine and total alkaloid in P. ternata were 0.0136% -0.0264% and 0.0155% -0.0652% respectively. Individual yield was 0.5536-2.9740 g. All of the populations could be classified into 3 types through hierarchical cluster analysis.</p><p><b>CONCLUSION</b>There exist significant differences in the content of guanosine, total content of alkaloid and individual yield of P. ternata from different populations. It is suggested that breeding and selection for type II of P. ternata should be strengthened.</p>
Subject(s)
Alkaloids , Breeding , China , Chromatography, High Pressure Liquid , Cluster Analysis , Ecosystem , Guanosine , Pharmacognosy , Economics , Pinellia , Chemistry , Classification , Plants, Medicinal , Chemistry , ClassificationABSTRACT
<p><b>OBJECTIVE</b>To provide theoretic warrant and technical reference for Salvia miltiorrhizr standardization planting, by carrying out various systemic studies such as observation of seeds configuration fabric, idiosyncrasy of water absorption and groping germinating conditions.</p><p><b>METHOD</b>In the study of configuration fabric, seeds were observed and taken photos by scanning electronic microscope, and heft method was used for measuring changes of water absorption velocity and dehydration velocity. Seeds germination conditions were probed into under the national test regulations for crop seeds and related prescription from international standards.</p><p><b>RESULT</b>(1) There was a layer of slime about 10-20 microm thickness covering epicarp of Danshen seeds. The slime formed as diamond meshwork (reseau) and the weight of it was 8%-10% of total seeds weight. (2) The speed of water absorption of seeds was extremely rapid. The weight of seeds could increase above 10 times as original while the dehydration velocity was quite low. (3) The optimal temperature for the seeds germination is around 25 degrees C, and the germination rate of the new seeds gained yearly was above 75%, but the rate would decrease sharply as years went by. It was also found that the seeds germination power and exponent of vigor were quite high under the temperature transformation between 23 degrees C, 28 degrees C. Such treatments as pre-cool, PEG treatment and infusing with GA3 could increase the rate of seeds germination capacity obviously.</p>